MACHEREY-NAGEL - Chromatography Consumable

Since 1911 MACHEREY-NAGEL stands for high quality, innovation and reliability in chemical and biomolecular analysis. Friendly expert advice for our highly valued customers as well as outstanding product quality have been the cornerstones of our corporate success for more than 100 years. MACHEREY-NAGEL is a family-owned company run by the fourth generation. As one of today’s leading manufacturers of products for analytical chemistry and life science we offer a broad range of products for Filtration, Rapid Tests, Water Analysis, Chromatography and Bioanalysis.

Headquarters and manufacturing site in Düren (Germany), further location in Oensingen (Switzerland)

 

MACHEREY-NAGEL has grown from a pioneer in chromatography to a full-range supplier of laboratory consumables. We supply laboratories all over the world with HPLC, GC and SPE columns, TLC plates and sheets, syringe filters or suitable vials and closures. Our philosophy includes personal and competent support as well as outstanding product quality. We have the demand to fulfill the customer’s individual needs and offer optimal and reliable solutions for your lab work in method development and routine analysis.

 

 

 

 

 

1. Solid phase extraction

Our CHROMABOND® QC policy

  • Highest production standard – our facilities are EN ISO 9001:2008 certified
  • All products are individually tested to meet our strict quality specifications, ensuring our outstanding product reproducibility, reliability and performance
  • Perfect reproducibility from lot-to-lot and within every single batch:
    > Careful attention to particle size distribution and pore diameters assures consistent column flow
    > Chemical reproducibility is guaranteed by strict quality control throughout manufacturing
  • Each product is supplied with a certificate of analysis stating the results of internal examinations and quality control

Design of columns, cartridges and 96-well plates

 

CHROMABOND® summary of MN phases

CHROMABOND®
Phase

Matrix

Modification / Application

Reversed phases

HR-X

PS/DVB

 

Easy

PS/DVB

polar, bifunctional

HR-P

PS/DVB

 

PS-RP

PS/DVB

removal of organic components

C18 ec

silica

octadecyl, endcapped

C18  ec f

silica

as above, fast flow

C18

silica

octadecyl, not endcapped

C18 f

silica

as above, fast flow

C18 Hydra

silica

octadecyl, not endcapped, for polar analytes

C8

silica

octyl

C4

silica

butyl

C2

silica

dimethyl

C6H11 ec

silica

cyclohexyl, endcapped

C6H5

silica

phenyl

Normal phases

SiOH

silica

unmodified

NH2

silica

aminopropyl

OH (Diol)

silica

diol

CN

silica

cyano

HILIC

silica

zwitterionic  ammonium-sulfonic acid modification

Alox A

aluminum oxide

acidic

Alox N

aluminum oxide

neutral

Alox B

aluminum oxide

basic

Florisil®

magnesium silicate

 

PA

polyamide 6

 

Ion exchangers

SA                            

silica                 

benzenesulfonic acid cation exchanger (SCX)   

SB                       

silica                   

quaternary ammonium anion exchanger (SAX) 

PCA                       

silica                  

propylcarboxylic acid cation exchanger (WCX)

PSA**                                   

silica                  

propylsulfonic acid cation exchanger

HR-XC

PS/DVB

strong mixed mode cation exchanger for

 

 

basic analytes (MCX)

HR-XA

PS/DVB

strong mixed mode anion exchanger for

 

 

acidic analytes (MAX)

HR-XCW

PS/DVB

weak mixed mode cation exchanger for basic

 

 

analytes (WCX)

HR-XAW

PS/DVB

weak mixed mode anion exchanger for acidic

 

 

analytes (WAX)

PS-OH–

PS/DVB

strong anion exchanger in OH– form

PS-H+

PS/DVB

strong cation exchanger in H+  form

PS-Mix

PS/DVB

mixture of PS-OH–  and PS-H+

PS-Ag+

PS/DVB

strong cation exchanger in Ag+  form

PS-Ba2+

PS/DVB

strong cation exchanger in Ba2+  form

Phases for special applications

Drug

silica

bifunctional C8/SA, for enrichment of drugs from urine

Drug II

silica

bifunctional C8/SB, for extraction of THC and derivatives and of acidic analytes from biological fluids

Tetracycline

silica

special octadecyl phase, for enrichment of tetracyclines

HR-P-AOX

PS/DVB

for extraction of AOX from water

 

 

(DIN 38409 – H22)

C18 PAH

silica

special octadecyl phase, for enrichment of

 

 

PAHs from water

NH2/C18

silica

combination phase for enrichment of PAHs

 

 

from water

CN/SiOH

silica

combination phase for enrichment of PAHs

 

 

from soil

Na2SO4/Florisil®

 

combination phase for extraction of hydrocar- bons from water (DIN H-53 / ISO DIS 9377-4)

NAN

silica /

combination phase for enrichment of PCBs

 

AgNO3 +  Na2SO4 

from sludge

SA/SiOH

silica

combination phase for enrichment of PCBs

 

 

from waste oil

SiOH-H2SO4/SA

silica

combination phase, used together with SiOH

 

 

for enrichment of PCB from oil

QuEChERS /

silica

primary and secondary amine functions

Diamino

 

(PSA), for determination of pesticides in food

 

 

samples (QuEChERS method)

ABC18

silica

octadecyl, with ion exchange functions, for

 

 

acrylamide analysis

Carbon A

activated

determination of acrylamide from water ac-

 

carbon

cording to DIN 38413-6

PL

 

specially developed SPE phase for the prepa-

 

 

ration of bioanalytical samples

Dry

Na2SO4

for drying organic samples

PTL/PTS

special mem-

phase separation

 

brane

 

XTR

kieselguhr

liquid-liquid extraction

 

* Phases which provide a similar selectivity based on chemical or physical properties (list not complete)
** For primary and secondary amine functions see QuEChERS / Diamino


CHROMABOND® QuEChERS special silica phase for determination of pesticides in food samples

 

CHROMABOND® PL special phase for removal of phospholipids

CHROMABOND® PTS and PTL PTS and PTL columns for phase separation

CHROMABOND® XTR for liquid-liquid extraction

SPE vacuum manifolds and accessories

For the simultaneous preparation of up to 12, 16 or 24 samples


2. Sample filtration

CHROMAFIL® – Ideal for GC, HPLC and UHPLC sample filtration By using CHROMAFIL® syringe filters unwanted particles are removed which can otherwise cause contamination and clogging of sensitive instrumentation. So, filtering your samples leads to an increase of lifetime and less downtime of chromatographic columns and equipment, gives more consistent and reproducible results and thereby saves time and money.

Features

  • Different membrane types to meet multiple filtration needs
  • Low content of extractable compounds
  • Fast flow geometry for easy filtration
  • Low hold-up volume for maximum filtrate recovery
  • HPLC certified
  • Designed to be compatible for the use on all common automated filtration systems, e.g., SOTAX® dissolution systems

CHROMAFIL® Xtra

Labeled for method validation and certification

  • Xtra  imprint for the direct identification of the membrane type, diameter and pore size
  • Xtra  low bleeding PP housing
  • Xtra  color-free plain polypropylene


3. Liquid chromatography

NUCLEODUR® high purity silica for HPLC

NUCLEODUR® is a fully synthetical type B silica (silica of 3rd
generation) offering highly advanced physical properties like
totally spherical particle shape, outstanding surface microstructure,
high pressure stability and low metal content.

NUCLEODUR® as a state-of-the-art silica is the ideal
base material for modern HPLC phases. It is the result of
MACHEREY-NAGEL’s pioneering research in chromatography
for more than 40 years.

Summary of available NUCLEODUR® phases

NUCLEOSHELL® core-shell silica for HPLC

Benefits of core-shell technology

Short diffusion paths

  • Rapid mass transfer (C term of the Van Deemter equation)
  • High flow velocity without peak broadening for fast LC

Narrow particle size distribution (d90/d10 ~ 1.1)

  • Stable packing

High heat transfer

  • Minimized influence of frictional heat
  • Column efficiency of NUCLEOSHELL® ~ 250000/m (HETP ~ 4 µm)

 

Column Protection System

Innovative and universal screw-on guard column holder system
Suitable for all analytical HPLC columns with 1/16" fittings

Features

  • Cartridges filled with specified NUCLEODUR®, NUCLEOSIL® and NUCLEOSHELL® HPLC adsorbents
  • Ideal protection for your analytical main column -> significant increase in column lifetime
  • Minimized void volume -> suitable also for ultra fast HPLC
  • Special ferrules -> pressure stability up to 1300 bar (18 850 psi)
  • Visual contamination check -> in-time changing of the guard column
  • Guard column length 4 mm, ID 2 mm (for main columns with 2 mm ID) or ID 3 mm (for main columns with 3, 4 and 4.6 mm ID)
  • UNIVERSAL RP guard columns suitable for all HPLC columns under RP conditions

4. Thin layer chromatography

Advantages of MN plates and sheets for TLC

Continuous high quality
•    Guaranteed by stringent production control including stan- dardized lot tests, surface checks for roughness or cracks as well as hardness and adherence checks

Comprehensive range of phases for TLC / HPTLC
•    There is no universal TLC plate which meets all possible types of analyses
•    Our versatile range of TLC ready-to-use layers covers many different types of applications

Immediately ready for chromatographic separation
•    Coatings or impregnations are not necessary

Homogeneous, smooth, well adhering layers
•    An important criterion especially for reproducible quantitative evaluation

Electron microscope photograph of a cross section through a glass plate with silica layer (magnification x 500)

 


5. Gas chromatography

Summary of MN phases for GC

Overview of OPTIMA® MN phases

Phase

Composition

max.
Temperature
[°C] *

Polarity

OPTIMA® 1

dimethylpolysiloxane

340 / 360

lowest

OPTIMA® 1 MS

dimethylpolysiloxane with low bleeding

340 / 360

 

OPTIMA® 1 MS Accent

dimethylpolysiloxane with ultra-low bleeding

340 / 360

OPTIMA® 5

5 % phenyl – 95 % methylpolysiloxane

340 / 360

OPTIMA® 5 MS

5 % diphenyl – 95 % dimethylpolysiloxane with low bleeding

340 / 360

OPTIMA® 5 MS Accent

silarylene phase with ultra-low bleeding: polarity similar to 5 % diphenyl – 95 % dimethylpolysiloxane

340 / 360

OPTIMA® 5 HT

cross-linked silarylene phase: polarity index according to 5 % phenyl – 95 % methylpolysiloxane

380 / 400

OPTIMA® XLB

silarylene phase with ultra-low bleeding:
optimized silarylene content

340 / 360

OPTIMA® δ-3

autoselective polarity between OPTIMA® 5 and OPTIMA® 1701

340 / 360

OPTIMA® δ-6

autoselective polarity between OPTIMA® 17 and OPTIMA® 210

340 / 360

OPTIMA® 1301

6 % cyanopropylphenyl – 94 % dimethylpolysiloxane

300 / 320

OPTIMA® 1301 MS

silarylene phase with very low bleeding: polarity similar to
6 % cyanopropylphenyl – 94 % dimethylpolysiloxane

300 / 320

OPTIMA® 624

6 % cyanopropylphenyl – 94 % dimethylpolysiloxane

280 / 300

OPTIMA® 624 LB

6 % cyanopropylphenyl – 94 % dimethylpolysiloxane, low bleed

280 / 300

OPTIMA® 1701

14 % cyanopropylphenyl – 86 % dimethylpolysiloxane

280 / 300

OPTIMA® 1701 MS

silarylene phase with very low bleeding: polarity similar to 14 % cyanopropylphenyl – 86 % dimethylpolysiloxane

280 / 300

OPTIMA® 35 MS

silarylene phase with very low bleeding: polarity similar to 35 % diphenyl – 65 % dimethylpolysiloxane

360 / 370

OPTIMA® 17

phenylmethylpolysiloxane (50 % phenyl)

300 / 320

OPTIMA® 17 MS

silarylene phase with very low bleeding: polarity similar to 50 % diphenyl – 50 % dimethylpolysiloxane

340 / 360

OPTIMA® 210

trifluoropropylmethylpolysiloxane (50 % trifluoropropyl)

260 / 280

OPTIMA® 225

50 % cyanopropylmethyl – 50 % phenylmethylpolysiloxane

260 / 280

OPTIMA® 240

33 % cyanopropylmethyl – 67 % dimethylpolysiloxane

260 / 280

OPTIMA® WAX

polyethylene glycol 20000 dalton

240 / 250

OPTIMA WAXplus®

polyethylene glycol with optimized crosslinking

260 / 270

 

OPTIMA® FFAP

polyethylene glycol-2-nitroterephthalate

240 / 250

 

OPTIMA® FFAPplus

polyethylene glycol-2-nitroterephthalate with optimized crosslinking

250 / 260

highest

 OPTIMA® δ · phases with autoselectivity

Contact Us

Phoenix Scientific Co., Ltd.
50/93-94 Phaholyothin Road, Anusavaree, Bangkhen, Bangkok 10220 Thailand.

Tel. (662) 970 3666
Fax. (662) 970 3636
Email: info@phoenix-sci.com
Line ID: phoenixscientific20

Email Subscription

Name
Invalid Input

Email
Invalid Input

Invalid Input